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You are here:Home » Biochemicals » Biochem-Molecular Biology » X-Gal (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal)

X-Gal (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal)


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Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white.
Catalog #X1000
Purity (HPLC) 99%
Purity (TLC): Single Spot
Appearance: White lyophilized powder
Solubility: Soluble in DMF
Water (KF): 1%
Specific Rotation: -60º to -64º
Identity by I.R. or NMR: Conforms to structure
RNase, DNase or Proteases: None Detected
Quality Control (Blue-White Assay)[LacZ+]: Pale blue center/dense blue periphery
[LacZ-]: Faint blue center/white periphery
Storage and StabilityStore at -20°C. Stable for 6 months. Protect from light.
Also AvailableX1000-05: XGAL, 40mg/ml in DMF
CAS Number7240-90-6
Molecular FormulaC14H15BrClNO6
Molecular Weight408.61
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names(X-Gal, 5-Bromo-4-chloro-3-indolyl-b-D-galactoside (galactopyranoside))

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