(X-Gal, 5-Bromo-4-chloro-3-indolyl-b-D-galactoside (galactopyranoside))

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Specifications

Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white.

Catalog #	X1000
Purity (HPLC)	99%
	Purity (TLC): Single Spot
	Appearance: White lyophilized powder
	Solubility: Soluble in DMF
	Specific Rotation: -60º to -64º
	Identity by I.R. or NMR: Conforms to structure
	RNase, DNase or Proteases: None Detected
Quality Control (Blue-White Assay)	[LacZ+]: Pale blue center/dense blue periphery
	[LacZ-]: Faint blue center/white periphery
Storage and Stability	Store at 4°C. Stable for at least 6 months. Protect from light.
Also Available	X1000: XGAL
	X1000-05: XGAL, 40mg/ml in DMF
CAS Number	[7240-90-6]
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.61
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	(X-Gal, 5-Bromo-4-chloro-3-indolyl-b-D-galactoside (galactopyranoside))

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