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Specifications

Indigo-blue chromogenic substrate used in the detection of beta-glucuronidase (GUS fusion marker) in E. coli for transcription and translation studies. Additional applications include bacterial contamination testing in water, meat, dairy, shellfish.
Catalog #	X1025
	The chromogenic substrate X-Gluc is used in a variety of applications for the detection of the B-glucuronidase enzyme. Upon reduction, X-Gluc produces a localized color, making it useful in identifying GUS gene presence in most cell types1 and for the detection of the GUS gene fusion marker in plants.2,3 X-Gluc has reported applications in the detection of contaminated foodsamples such as meat, dairy, and shellfish products.4,5 X-Gluc also has clinical applications in the assessment of urinary tract infection by d etecting the pr esence of E. coli.6 It has gained international acceptance as an accurate indicator for the presence of E. coli in potable water samples b y reducing false positives and negatives found with traditional methods.
	BENEFITS-Saves time compared to other E. coli detection methods... 24 hour direct plating method7,9,11-Minimizes errors...less than 1% false negatives and less than 5% false positives7,8,9-Easy visual d etection...8max is in visible range-Sensitive for p resence/ab sence of E. coli...eliminates limitation s of very rapid presence/absence or chemiluminometric methods12
APPLICATIONS	Suitable for use in environmental testing of E. coli in potable water, detection of GUS expression in the study of trans cription, translation, and protein transport events, determination of urinary tract infections and identifying food products contaminated with E. coli
CAS Number	114162-64-0
Molecular Formula	C20H26BrClN2O7
Molecular Weight	521.79
• Purity	99%
• Water (KF)	5.0%
• Specific Rotation	-86º to -90º
• RNase	None Detected
• DNase	None Detected
• Protease	None Detected
Alternate names	5-Bromo-4-chloro-3-indolyl-?-D-glucuronide cyclohexylammonium salt