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You are here:Home » Molecular Biology » MB-Enzymes, Substrates » XGLUC, Sodium Salt

XGLUC, Sodium Salt


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Indigo-blue chromogenic substrate used in the detection of -glucuronidase in bacterial colonies.
Catalog #X1027
The chromogenic substrate X-Gluc is used in a variety of applications for the detection of the $-glucuronidase enzyme. Upon reduction, X-Gluc produces a localized color, making it useful in identifying GUS gene presence in most cell types1 and for the detection of the GUS gene fusion marker in plants.2,3 X-Gluc has reported applications in the detection of contaminated food samples such as meat, dairy, and shellfish products.4,5 X-Gluc also has clinical applications in the assessment of urinary tract infection by d etecting the pr esence of E. coli.6 It has gained international acceptance as an accurate indicator for the presence of E. coli in potable water samples b y reducing false positives and negatives found with traditional methods.
BENEFITS -Saves time compared to other E. coli detection methods... 24 hour direct plating method7,9,11 -Minimizes errors...less than 1% false negatives and less than 5% false positives7,8,9 -Easy visual d etection...8max is in visible range -Sensitive for p resence/ab sence of E. coli...eliminates limitation s of very rapid presence/absence or chemiluminometric methods12
ApplicationsSuitable for use in environmental testing of E. coli in potable water, detection of GUS expression in the study of trans cription, translation, and protein transport events, determination of urinary tract infections and identifying food products contaminated with E. coli
Specific Rotation (C=1, H2ODMF 1:1): -107 to -110°
Solubility (2% DMF): Colorless, clear, complete
Water (KF): ~10%
H-NMR: Conform to structure
DNase or Proteases:
None Detected
CAS Number129541-41-9
Molecular FormulaC14H12BrClNNaO7
Molecular Weight444.7
FormWhite crystalline powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names5-Bromo-4-chloro-3-indolyl-?-D-glucuronide sodium salt

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