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Specifications

Indigo-blue chromogenic substrate used in the detection of -glucuronidase in bacterial colonies.
Catalog #	X1027
	The chromogenic substrate X-Gluc is used in a variety of applications for the detection of the $-glucuronidase enzyme. Upon reduction, X-Gluc produces a localized color, making it useful in identifying GUS gene presence in most cell types1 and for the detection of the GUS gene fusion marker in plants.2,3 X-Gluc has reported applications in the detection of contaminated foodsamples such as meat, dairy, and shellfish products.4,5 X-Gluc also has clinical applications in the assessment of urinary tract infection by d etecting the pr esence of E. coli.6 It has gained international acceptance as an accurate indicator for the presence of E. coli in potable water samples b y reducing false positives and negatives found with traditional methods.
	BENEFITS-Saves time compared to other E. coli detection methods... 24 hour direct plating method7,9,11-Minimizes errors...less than 1% false negatives and less than 5% false positives7,8,9-Easy visual d etection...8max is in visible range-Sensitive for p resence/ab sence of E. coli...eliminates limitation s of very rapid presence/absence or chemiluminometric methods12
Applications	Suitable for use in environmental testing of E. coli in potable water, detection of GUS expression in the study of trans cription, translation, and protein transport events, determination of urinary tract infections and identifying food products contaminated with E. coli
Specific Rotation (C=1, H2O	DMF 1:1): -107 to -110°
	Solubility (2% DMF): Colorless, clear, complete
	Water (KF): ~10%
	H-NMR: Conform to structure
	DNase or Proteases:
	None Detected
CAS Number	129541-41-9
Molecular Formula	C14H12BrClNNaO7
Molecular Weight	444.7
Purity	98%
Form	White crystalline powder.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	5-Bromo-4-chloro-3-indolyl-?-D-glucuronide sodium salt