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You are here:Home » Culture Media » Media-Yeast » Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder)

Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder)

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Specifications

Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.
Catalog #Y2025
Yeast Nitrogen Base without amino acids (YNB w/o AA), Y2025, is used for selecting yeasts based on amino acid and carbohydrate requirements. In Y2025, Ammonium Sulfate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells. Compared to full YNB medium, Y2025 lacks Histidine, Methionine and Trypthophan. In combination with “Drop Out” medium supplement mixtures, Y2025 provides an excellent medium to cultivate and select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins.
ApplicationFor carbon assimilation tests in the characterization and identification of yeasts.
Note The addition of a carbon source like glucose, galactose or raffinose is required.
Appearance
White to off-white, homogeneous, free-flowing powder
Solubility
Colorless to pale yellow, clear, complete
pH
4.5±1.0
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesWickerham formula without amino acids & with ammonium sulfate


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