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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -Yes Kinase (Src p62 Non-receptor Tyrosine Kinases)

Anti -Yes Kinase (Src p62 Non-receptor Tyrosine Kinases)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Yes is a member of the Src family of non-receptor tyrosine kinases and amplifies signals generated by various receptors. Expression of Yes is elevated in melanocytes and in melanoma cells. Yes activity is stimulated by neurotrophins, which are mitogenic and metastatic factors for melanoma cells. Yes is essential for MAP Kinase activation by angiotensin II.
Catalog #Y2054
ApplicationsSuitable for use in Western Blot. Not recommended for Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects Yes Kinase in RIPA lysates from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Yes (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Positive Antigen Control J9000
Jurkat cell lysate. Use 20ug per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenTrpE fusion protein corresponding to aa 1-252 of human Yes Kinase.
SpecificityRecognizes human p62 Yes Kinase, which occasionally appears as a doublet of 59kD and 62kD. Species Crossreactivity: human, mouse and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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