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You are here:Home » Antibodies » Antibodies-Carbohydrates, Glycoproteins » Anti -ZAG (Zinc-a2-Glycoprotein 1)

Anti -ZAG (Zinc-a2-Glycoprotein 1)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
ZAG (zinc-a2-glycoprotein; also ZA2G) is a 40kD, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26-201) and a C2-type Ig-like domain (aa 207-292). Two alternate splice forms exist; one shows a 66 aa substitution for the C-terminal 30 aa, and a second shows a nine Lys substitution for aa 151-298. Mature human ZAG is 60% aa identical to mouse ZAG.
Catalog #Z0133
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5 -1.0ug/ml detection limit for rhZAG is ~ 0.3ng/well.
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived rhZAG. (aa 21 - 298)
SpecificityRecognizes human ZAG.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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