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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

ZAG (zinc-a2-glycoprotein; also ZA2G) is a 40kD, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26-201) and a C2-type Ig-like domain (aa 207-292). Two alternate splice forms exist; one shows a 66 aa substitution for the C-terminal 30 aa, and a second shows a nine Lys substitution for aa 151-298. Mature human ZAG is 60% aa identical to mouse ZAG.
Catalog #	Z0133
Applications	Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Direct ELISA: 0.5 -1.0ug/ml detection limit for rhZAG is ~ 0.3ng/well.
	Western Blot: 1.0ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	NS0-derived rhZAG. (aa 21 - 298)
Specificity	Recognizes human ZAG.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.