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Z0400-10M Goat Anti-ZASP (LIM Domain-binding Protein 3, Protein Cypher, Z-band Alternatively Spliced PDZ-motif Protein, LDB3, KIAA0613)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Ca Hu Mo Po Rt
Accession #
NP_009009.1, NP_001073583.1
Gene ID
11155
Shipping Temp
Blue Ice
Storage Temp
-20°C

May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1000 Western Blot: 0.1-1ug/ml, in human skeletal muscle lysate on ~90, 35kD bands Immunohistochemistry (Paraffin): 3-6ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-KSQNKPEDEADE, from human LDB3, at the internal region of the protein (NP_009009.1, NP_001073583.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human LDB3. Species sequence homology: Canine, mouse, porcine and rat.
References
1. Selcen D, Engel AG. Mutations in ZASP define a novel form of muscular dystrophy in humans. Ann Neurol. 2005 Feb;57(2):269-76.
USBio References
No references available
Conjugates
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