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Z0744-36B Goat Anti-ZNF364 (E3 Ubiquitin-protein Ligase RNF115, RING Finger Protein 115, Rabring 7, Zinc Finger Protein 364, RNF115)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Ca Hu Mo Rt
Accession #
NP_055270.1
Gene ID
27246, 67845, 362002
Shipping Temp
Blue Ice
Storage Temp
-20°C

Acts as an E2-dependent E3 ubiquitin-protein ligase. May be involved in endocytic trafficking.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Western Blot: 0.3-1ug/ml, observed in human lysates of cell line HEPG2 on ~35kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-DQDNRANERGHQTH, from human RNF115, at the internal region of the protein (NP_055270.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human RNF115. Species sequence homology: Canine, mouse and rat.
References
1. Burger AM, Gao Y, Amemiya Y, Kahn HJ, Kitching R, Yang Y, Sun P, Narod SA, Hanna WM, Seth AK. A novel RING-type ubiquitin ligase breast cancer-associated gene 2 correlates with outcome in invasive breast cancer. Cancer Res. 2005 Nov 15;65(22):10401-12.
USBio References
No references available
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