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Anti -ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1) (FITC)
Anti -ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1) (FITC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IF |
|
| Tight junctions (or zonula occludens) are selective, semi-permeable barriers in the paracellular pathway of vertebrate endothelia and epithelia. Tight junctions also serve to physically separate functionally and biochemically distinct regions of the plasma membrane that exist in distinct physiological compartments (1). ZO-1, ZO-2, ZO-3 and occludin are major components of vertebrate tight junctions. ZO-1 is a peripheral (non-transmembrane) membrane protein found on the cytoplasmic leaflet of tight junctions. ZO-1 has a MW of 225kD. It is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as ‘motif-a’ (2) and constitute a basis for classifying tight junction (3). ZO-1 is homologous to the dlg (discs-large) protein of Drosophila and the C. elegans lin-2 gene product. Loss of dlg results in neoplastic overgrowth of imaginal discs and loss of epithelial apical/basal polarity. ZO-1 and the interacting protein ZO-2 are members of a family of putative signal transduction proteins (MAGUK) whose members contain an SH3 domain, a domain with homology to guanlylate kinases and a PDZ domain (potential mediator of protein-protein interaction) found in many synapse-associated proteins (4,5). | | | Catalog # | Z0750-01 | | Applications | Suitable for use in Direct Immunofluorescence. Other applications not tested. | | Recommended Dilution | Direct Immunofluorescence: 5-10ug/ml | | Optimal dilutions to be determined by the researcher. | | Source | Ascites | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgGl,k | | Clone No | 3G302 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide, 40% glycerol. Labeled with fluorescein isothiocyanate (FITC). | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Fusion protein cooresponding to aa334-634 of human ZO-1. | | Specificity | Recognizes human ZO-1a+ and ZO-1a- isoforms. The antibody is specific for ZO-1 proteins. Crossreactivity with the related ZO-2 protein has not been observed. Species Crossreactivity: canine. Confirmed with T84 (human colon), Caco-2 cells (human colon) and MDCK (canine kidney) cells. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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