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Anti -ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1) (Glycerol Free)
Anti -ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1) (Glycerol Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IF |
|
| Tight junctions (or zonula occludens) are selective, semi-permeable barriers in the paracellular pathway of vertebrate endothelia and epithelia. Tight junctions also serve to physically separate functionally and biochemically distinct regions of the plasma membrane that exist in distinct physiological compartments. ZO-1, ZO-2 and occludin are major components of vertebrate tight junctions. ZO-1 is a peripheral (non-transmembrane) membrane protein found on the cytoplasmic leaflet of tight junctions. ZO-1 has a MW of 225kD and is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as 'motif-a' and constitute a basis for classifying tight junctions. ZO-1 is homologous to the dlg (discs-large) protein of Drosophila and the C. elegans lin-2 gene product. Loss of dlg results in neoplastic overgrowth of imaginal discs and loss of epithelial apical/basal polarity. ZO-1 and the interacting protein ZO-2 are members of a family of putative signal transduction proteins (MAGUK) whose members contain an SH3 domain, a domain with homology to guanlylate kinases and a PDZ domain (potential mediator of protein-protein interaction) found in many synapse-associated proteins. | | | Catalog # | Z0750-02 | | Applications | Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications have not been tested. | | Recommended Dilutions | ELISA: 0.1-1ug/ml | | Immunofluorescence: 5-10ug/ml | | Western Blot: 1-2ug/ml | | Optimal dilutions to be determined by the researcher. | | Recommended Positive Controls | Caco-2 and MDCK cells | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | N/A | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 6A218 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Glycerol Free. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant protein corresponding to aa334-634 of human ZO-1. | | Specificity | Recognizes human ZO-1a+ and ZO-1a- isoforms. Crossreactivity with the related ZO-2 protein has not been observed. | | Species Crossreactivity | Canine | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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