| ZO-1 is a peripheral membrane protein that is associated with tight junctions (zonula occludentes) which are found on the cytoplasmic surface of epithelial and endothelial cell membranes. ZO-1 has a MW of 225kD and is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as 'motif-a'. These two isoforms are the basis for determining two classes of tight junctions. ZO-1 is known to interact with ZO-2. ZO-1 is homologous to dlg, the Drosophila tumor suppressor. Both are members of a putative signal transduction protein family whose members contain a SH3 domain and a domain homologous to the enzyme guanylate kinase. |
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| Catalog # | Z0750-02B |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 0.5-2ug/ml |
| Immunoprecipitation: 2-5ug/IP reaction |
| Immunohistochemistry (Paraffin/Frozen): 2-5ug/ml. To achieve appropriate staining results on formalin-fixed, paraffin-embedded tissue sections, protease pretreatment is required. Use 1-2mg/ml protease and incubate at 37°C for 10 minutes. Other enzyme and HIER pre-treatment methods may not be suitable for this antibody. Frozen sections have been successfully stained after fixation with acetone at 4°C. |
| Optimal dilutions to be determined by the researcher. |
| Positive Controls for Immunoprecipitation | Canine MDCK and human Caco-2 cells. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.25mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies from the N-terminal to the 80 amino acid region (the alpha-motif) present in the a+isoform but absent in the alpha- isoform due to alternative splicing. |
| Specificity | Recognizes human ZO-1 alpha+ and ZO-1 alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western Blots, but potential reactivity with these proteins has not been fully characterized. Species Crossreactivtiy: mouse, rat, guinea pig, canine. Moderate reactivity with chicken. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
