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You are here:Home » Antibodies » Antibodies-Connexin, Junction Proteins » Anti -ZO-1 (Mid) (Tight Junction-associated Polypeptide, Zonula Occludens 1)

Anti -ZO-1 (Mid) (Tight Junction-associated Polypeptide, Zonula Occludens 1)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IF
The tight junction is a cell-to-cell adhesion structure in epithelial cells that constitute the epithelial junctional complex with adherens junctions and desmosomes. Tight junctions seal cells to create a primary barrier to the diffusion of solutes across the cellular sheet and also function as a boundary between the apical and basolateral membrane domains to produce their polarization.1 Tight junction strands are mainly composed of claudins, occludin, and JAM.2 Various scaffold proteins have been reported to be concentrated at the cytoplasmic surfaces of the junctional complex regions to determine the specialization and localization of junctions: ZO-1, ZO-2, and ZO-3. The zona occludins (ZO) proteins constitute the plaque structures underlying plasma membranes together with various proteins including cingulin, symplekin, Par-3·Par-6·atypical protein kinase C complex, ZONAB, and guanine nucleotide exchange factor-H1/Lfc.2 All three ZO proteins have three PDZ domains, one Src homology 3 domain, and one guanylate kinase-like homologue domain in this order from their NH2 termini, indicating that ZO-1, ZO-2, and ZO-3 are membrane-associated guanylate kinase-like homologues (MAGUKs).
Catalog #Z0750-02D
ApplicationsSuitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1-3ug/ml
Immunofluorescence: 2.5ug/ml
Immunohistochemistry (Frozen): 2ug/ml with frozen mouse liver, blood vessel (heart) and mossy fiber terminal (brain) tissues
Optimal dilutions to be determined by the researcher.
Positive Control Canine MDCK-II, human A431, Caco-2, rat Rat-1 and NRK-52E cell lysates by WB.
MDCK-II, Caco-2, Rat-1 and NRK-52E cells by IF.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the mid region of human ZO-1, which differs from canine, mouse, and rat by one conservative amino acid replacement.
SpecificityRecognizes human ZO-1 protein at ~225kD. Bands of unknown origin may be observed at lower molecular weights in some sample lysates. These bands may represent breakdown products.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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