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You are here:Home » Antibodies » Antibodies-Connexin, Junction Proteins » Anti -ZO-1 (Tight junction protein 1, Tight junction protein ZO-1, ZO1, ZO-1, Zona Occludens 1 Protein, Zonula Occludens 1 Protein)

Anti -ZO-1 (Tight junction protein 1, Tight junction protein ZO-1, ZO1, ZO-1, Zona
Occludens 1 Protein, Zonula Occludens 1 Protein)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B FC IF
Human zona occludens 1 (ZO-1), an ~220kD tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes.
Catalog #Z0750-02E
ApplicationsSuitable for use in Immunofluorescence, Western Blot, and Flow Cytometry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:50. Intracellular staining is required. Cells were fixed with 4% paraformaldehyde and cells were permeabilized with PBS, 0.1% saponin and 0.5% BSA.
Western Blot: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenGST-ZO-1 (aa432-1150).
SpecificityRecognizes both ZO-1 alpha-minus and alpha-plus human zona occludens 1 (ZO-1). Species crossreactivity: mouse, dog
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesTJP1

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