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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

Anti -ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The ZO-1 and ZO-2 proteins share significant sequence homology to each other (51% identity), as well as to several other proteins which include the lethal(1)discs-large(dlg) tumor supressor gene product (dlg-A) of Drosophila, erythrocyte membrane-associated p55 protein, and PSSD-95/SAP90. These proteins share several conserved domains and are collectively referred to as the MAGUK family. The regions of homology among this family of proteins include a domain homologous to guanylate kinase (GUK), a single SH3 domain, and a variable number of N-terminal repeated domains termed PDZ domains. The ZO-1 and ZO-2 proteins are distinct from the other family member in that they possess C-terminal acidic and proline rich regions, and alternatively spliced domains at their C-termini. Although, ZO-1 and ZO-2 are structurally similar and display significant sequence homology, the proteins are divergent at their C-termini. This suggests that the C-terminal ends of ZO-1 and ZO-2 have distinct functions. The canine ZO-2 protein has a predicted molecular mass of ~132kD. However, the protein migrates with a mass of 160kD when analyzed by SDS-PAGE.
Catalog #Z0760
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 0.1-1ug/ml
Western Blot: 1-4ug/ml
Immunohistochemistry (Mouse liver tissues): 4-5ug/ml
Note: Weak fixation of tissues with 2% paraformaldehyde/picric acid followed by NO post-fixation is required to produce staining.
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa and canine MDCK cell lysates and mouse liver homogenates.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceCanine
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the central portion of the ZO-2 protein.
SpecificitySpecific for the canine ZO-2 protein (~160kD). Does exhibit crossreactivity with a few proteins of unknown identity in the 50-85kD region. Does not appear to crossreact with the related ZO-1 protein. Species crossreactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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