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Anti -ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)
Anti -ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| The ZO-1 and ZO-2 proteins share significant sequence homology to each other (51% identity), as well as to several other proteins which include the lethal(1)discs-large(dlg) tumor supressor gene product (dlg-A) of Drosophila, erythrocyte membrane-associated p55 protein, and PSSD-95/SAP90. These proteins share several conserved domains and are collectively referred to as the MAGUK family. The regions of homology among this family of proteins include a domain homologous to guanylate kinase (GUK), a single SH3 domain, and a variable number of N-terminal repeated domains termed PDZ domains. The ZO-1 and ZO-2 proteins are distinct from the other family member in that they possess C-terminal acidic and proline rich regions, and alternatively spliced domains at their C-termini. Although, ZO-1 and ZO-2 are structurally similar and display significant sequence homology, the proteins are divergent at their C-termini. This suggests that the C-terminal ends of ZO-1 and ZO-2 have distinct functions. The canine ZO-2 protein has a predicted molecular mass of ~132kD. However, the protein migrates with a mass of 160kD when analyzed by SDS-PAGE. | | | Catalog # | Z0760 | | Applications | Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. | | Recommended Dilutions | ELISA: 0.1-1ug/ml | | Western Blot: 1-4ug/ml | | Immunohistochemistry (Mouse liver tissues): 4-5ug/ml | | Note: Weak fixation of tissues with 2% paraformaldehyde/picric acid followed by NO post-fixation is required to produce staining. | | Optimal dilutions to be determined by the researcher. | | Positive Control | HeLa and canine MDCK cell lysates and mouse liver homogenates. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Canine | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide derived from the central portion of the ZO-2 protein. | | Specificity | Specific for the canine ZO-2 protein (~160kD). Does exhibit crossreactivity with a few proteins of unknown identity in the 50-85kD region. Does not appear to crossreact with the related ZO-1 protein. Species crossreactivity: human and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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