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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

Anti -ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IF
The zonula occludens proteins (ZOPs), ZO-1, ZO-2 and ZO-3 are tight-junction phosphorylated proteins that belong to the membrane-associated guanylated kinase (MAGUK) family. ZOs are involved in the organization of epithelial and endothelial intercellular junctions and bind to the cytoplasmic C-termini of junctional transmembrane proteins linking them to the actin
cytoskeleton, ZOPs contain three PDZ domains, one SH3 domain, and a guanylate kinase-like domain, indicating that ZOPs may serve multiple purposes. ZOPs contain some unique motifs not shared by other MAGUK family members, including nuclear localization and nuclear export signals and a leucine zipper-like sequence. Their potential involvement in cell growing and proliferation has been suggested based on the observation that the N-terminal half of ZOPs displays a significant similarity to the product of the Drosophila tumor suppressor gene lethal disc-large (dlg).
Catalog #Z0760-01
ApplicationsSuitable for use in Immunoprecipitation, Western Blot, Immunofluorescence and ELISA. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Native): 5-10ug
Western Blot: 1-3ug/ml
Immunofluorescence: 1-3ug/ml
ELISA (Native): 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlsHuman CaCo2 and Canine MDCK cell lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6A219
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to internal amino acids of the human ZO-2 protein.
SpecificityRecognizes human ZO-2 at ~160kD. Species Crossreactivity: canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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