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Z0760-01 Mouse Anti-ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
6A219
Grade
Affinity Purified
Applications
E IF IP WB
Crossreactivity
Ca Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

The zonula occludens proteins (ZOPs), ZO-1, ZO-2 and ZO-3 are tight-junction phosphorylated proteins that belong to the membrane-associated guanylated kinase (MAGUK) family. ZOs are involved in the organization of epithelial and endothelial intercellular junctions and bind to the cytoplasmic C-termini of junctional transmembrane proteins linking them to the actin cytoskeleton, ZOPs contain three PDZ domains, one SH3 domain, and a guanylate kinase-like domain, indicating that ZOPs may serve multiple purposes. ZOPs contain some unique motifs not shared by other MAGUK family members, including nuclear localization and nuclear export signals and a leucine zipper-like sequence. Their potential involvement in cell growing and proliferation has been suggested based on the observation that the N-terminal half of ZOPs displays a significant similarity to the product of the Drosophila tumor suppressor gene lethal disc-large (dlg).

Applications
Suitable for use in Immunoprecipitation, Western Blot, Immunofluorescence and ELISA. Other applications have not been tested.
Recommended Dilution
Immunoprecipitation (Native): 5-10ug Western Blot: 1-3ug/ml Immunofluorescence: 1-3ug/ml ELISA (Native): 0.1-1ug/ml Optimal dilutions to be determined by the researcher.
Positive Controls
Human CaCo2 and Canine MDCK cell lysates
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to internal amino acids of the human ZO-2 protein.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ZO-2 at ~160kD. Species Crossreactivity: canine.
References
1. Beatch M, et al. J of Biol Chem 271(42): 25723 – 25726, 1996.2. Anderson JM, et al. Annu Rev Physiol 60:121-142, 1998.3. Wirrchen E, et al. J Biol Chem 274(49):35179-35185, 1999.4. Traweger A, et al. J of Biol Chem 271(42): 2692 – 2700, 2003
USBio References
No references available
Conjugates
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