| The zonula occludens proteins (ZOPs), ZO-1, ZO-2 and ZO-3 are tight-junction phosphorylated proteins that belong to the membrane-associated guanylated kinase (MAGUK) family. ZOs are involved in the organization of epithelial and endothelial intercellular junctions and bind to the cytoplasmic C-termini of junctional transmembrane proteins linking them to the actin |
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| cytoskeleton, ZOPs contain three PDZ domains, one SH3 domain, and a guanylate kinase-like domain, indicating that ZOPs may serve multiple purposes. ZOPs contain some unique motifs not shared by other MAGUK family members, including nuclear localization and nuclear export signals and a leucine zipper-like sequence. Their potential involvement in cell growing and proliferation has been suggested based on the observation that the N-terminal half of ZOPs displays a significant similarity to the product of the Drosophila tumor suppressor gene lethal disc-large (dlg). |
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| Catalog # | Z0760-01 |
| Applications | Suitable for use in Immunoprecipitation, Western Blot, Immunofluorescence and ELISA. Other applications have not been tested. |
| Recommended Dilution | Immunoprecipitation (Native): 5-10ug |
| Western Blot: 1-3ug/ml |
| Immunofluorescence: 1-3ug/ml |
| ELISA (Native): 0.1-1ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Positive Controls | Human CaCo2 and Canine MDCK cell lysates |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 6A219 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to internal amino acids of the human ZO-2 protein. |
| Specificity | Recognizes human ZO-2 at ~160kD. Species Crossreactivity: canine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
