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You are here:Home » Antibodies » Antibodies-Connexin, Junction Proteins » Anti -ZO-2, Mid (Tight Junction-associated Polypeptide, Zonula Occludens 2)

Anti -ZO-2, Mid (Tight Junction-associated Polypeptide, Zonula Occludens 2)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IF
Tight junctions are intercellular junctions that act as both the barrier (or gate), and the fence. The barrier function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces. The fence function maintains cell polarity, thus, tight junctions work as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane. Tight junctions are comprised of the membrane proteins ZO, occludin, claudins, and JAMs. The zonula occludens proteins, ZO-1, ZO-2 and ZO-3 are tight junction proteins that belong to the membrane-associated guanylated kinase (MAGUK) family. ZOs are involved in the organization of epithelial and endothelial intercellular junctions and bind to the cytoplasmic C-termini of junctional transmembrane proteins linking them to the actin cytoskeleton. ZOs contain some unique motifs not shared by other MAGUK family members, including nuclear localization and nuclear export signals and a leucine zipper-like sequence. ZOs also have potential involvement in cell growth and proliferation.
Catalog #Z0760-01B
ApplicaitonsSuitable for use in Western Blot and Immunofluorescence. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Immunofluorescence: 2.5ug/ml
Important: Cells were fixed with ice-cold ethanol for 30 minutes on ice prior to immunofluorescent assays.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the middle region of human ZO-2.
SpecificityRecognizes the human ~130-160kD ZO-2 protein. This antibody will recognize human ZO-2 isoforms A or C. Reactivity has been confirmed with CaCO2 cell lysates. No cross-reactivity with rat, canine, or mouse in Western Blot or ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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