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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -ZO-2, CT (Tight Junction-associated Polypeptide, Zonula Occludens 2)

Anti -ZO-2, CT (Tight Junction-associated Polypeptide, Zonula Occludens 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IF
Tight junctions are intercellular junctions that act as both the barrier (or gate), and the fence. The barrier function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces. The fence function maintains cell polarity, thus, tight junctions work as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane. Tight junctions are comprised of the membrane proteins ZO, occludin, claudins, and JAMs. The zonula occludens proteins, ZO-1, ZO-2 and ZO-3 are tight junction proteins that belong to the membrane-associated guanylated kinase (MAGUK) family. ZOs are involved in the organization of epithelial and endothelial intercellular junctions and bind to the cytoplasmic C-termini of junctional transmembrane proteins linking them to the actin cytoskeleton. ZOs contain some unique motifs not shared by other MAGUK family members, including nuclear localization and nuclear export signals and a leucine zipper-like sequence. ZOs also have potential involvement in cell growth and proliferation.
Catalog #Z0760-01C
ApplicationsSuitable for use in Western Blot and Immunofluorescence. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1-3ug/ml
Immunofluorescence: 2.5ug/ml. Cells need to be fixed with ice-cold ethanol for 30 minutes on ice prior to assays.
Optimal dilutions to be determined by the researcher.
Positive ControlsWestern Blot: CaCO2 human colorectal adenocarcinoma, MDCK II canine kidney, KNRK rat kidney, and TMCK-1 mouse kidney cell lysates..
Immunofluorescence: CaCO2, MDCK II, and NRK52E.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the C-terminal region of human or canine ZO-2.
SpecificityRecognizes the ~130-160kD human ZO-2 protein, isoforms A or C. A band of unknown origin lower than 75kD may be observed in certain lysates. Species Crossreactivity: rat, canine and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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