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Anti -ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)
Anti -ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Guinea pig |
Purified |
B IH IF |
|
| Human zona occludens 2 (ZO-2), an ~160kD tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-2, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. | | | Catalog # | Z0760-01D | | ZO-2 can interact with zona occludens 1 (ZO-1). Furthermore, the PDZ2 domain of ZO-2 was shown to interact with connexin-43, the predominant connexin in epithelial and most other tissues which is involved in cell growth control and embryonic development. | | Applications | Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:50 | | Western Blot: 1:50 | | Immunohistochemistry (frozen): 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Guinea pig | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. | | Purity | Purified | | Immunogen | ZO-2, aa 411-467. | | Specificity | Recognizes human zona occludens 2 (ZO-2), an ~160kD tight junction protein belonging | | to the membrane-associated guanlyate kinase (MAGUK) family. Species crossreactivity | Mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | TJP2 |
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