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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

Anti -ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

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Specifications

Clone Host Grade Applications
Polyclonal Guinea pig Purified B IH IF
Human zona occludens 2 (ZO-2), an ~160kD tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-2, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes.
Catalog #Z0760-01D
ZO-2 can interact with zona occludens 1 (ZO-1). Furthermore, the PDZ2 domain of ZO-2 was shown to interact with connexin-43, the predominant connexin in epithelial and most other tissues which is involved in cell growth control and embryonic development.
ApplicationsSuitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:50
Western Blot: 1:50
Immunohistochemistry (frozen): 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGuinea pig
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified
ImmunogenZO-2, aa 411-467.
SpecificityRecognizes human zona occludens 2 (ZO-2), an ~160kD tight junction protein belonging
to the membrane-associated guanlyate kinase (MAGUK) family. Species crossreactivityMouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesTJP2


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