Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -ZO-3, aa754-898 (Tight Junction-associated Polypeptide, Zonula Occludens 3)

Anti -ZO-3, aa754-898 (Tight Junction-associated Polypeptide, Zonula Occludens 3)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B IC IF
Tight junctions (or zonula occludens) are selective, semi-permeable barriers in the paracellular pathway of vertebrate endothelia and epithelia. Tight junctions also serve to physically separate functionally and biochemically distinct regions of the plasma membrane that exist in distinct physiological compartments.(1) ZO-1, ZO-2, ZO-3 and occludin are major components of vertebrate tight junctions. ZO-1 is a peripheral (non-transmembrane) membrane protein found on the cytoplasmic leaflet of tight junctions. ZO-1 has a MW of 225kD and is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as ‘motif-a’ (2) and constitute a basis for classifying tight junction.(3) ZO-1 is homologous to the dlg (discslarge) protein of Drosophila and the C. elegans lin-2 gene product. Loss of dlg results in neoplastic overgrowth of imaginal discs and loss of epithelial apical/basal polarity. ZO-1, and the interacting protein ZO-2 are members of a family of putative signal transduction proteins (MAGUK) whose members contain an SH3 domain, a domain with homology to guanlylate kinases and a PDZ domain (potential mediator of protein-protein interaction) found in many synapse-associated proteins.(4,5)
Catalog #Z0770
ApplicationsSuitable for use in Immunoblot and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionImmunoblotting: 3-10ug/ml using ECL
Immunocytochemistry: 10ug/ml for immunofluorescence on MDCK cell line fixed by acid ethanol (permeabilize with Triton X-100) or fixed with paraformaldehyde (permeabilize with Triton X-100).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceCanine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
PurityPurified IgG
ImmunogenZO-3 fusion protein (amino acids 754-898)
SpecificityZO-3 a 130kD protein which co-immunoprecipitates with the tight junction protein ZO-1of canine MDCK cell line.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links