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You are here:Home » Antibodies » Antibodies-Connexin, Junction Proteins » Anti -ZO-3, CT (Tight Junction-associated Polypeptide, Zonula Occludens 3)

Anti -ZO-3, CT (Tight Junction-associated Polypeptide, Zonula Occludens 3)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IF
ZO-3, a ~130kD protein, contains three PSD-95/SAP90, discs-large, ZO-1 (PDZ) domains, a SH3 domain, and a guanylate kinase-liked domain. Like ZO-1 and ZO-2, ZO-3 contains a C-terminal acidic domain and a basic region (between the first and second PDZ domains), making it belongs to the MAGUK protein family. By Western Blot analysis, ZO-3 is identified as a ~130kD protein that directly interacts with tight junction proteins ZO-1 and occuldin, but not with ZO-2. However, in one study, ZO-2, ZO-3 and Occludin colocalized with actin aggregates at cell border in MDCK cells. ZO-3 is localized to sites of cell-cell interaction, identical to that of ZO-1 in tight junctions. By immunoprecipitation analysis, ZO-3 co-immunoprecipitates with ZO-1. Using MDCK cells, ZO-3 protein colocalized with ZO-1 by immunofluorescence. In vitro binding assays, the N-terminal of ZO-3 showed preferably binding of ZO-1 and actin filaments (3). Moreover, the binding of ZO-1/ZO-2/ZO-3 was also found to interact with certain Claudin proteins.
Catalog #Z0773
ApplicationsSuitable for use in Immunoflourescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminus of the human ZO-3 protein.
SpecificityRecognizes the human ZO-3 protein. MW
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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