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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -ZO-3, CT (Tight Junction-associated Polypeptide, Zonula Occludens 3)

Anti -ZO-3, CT (Tight Junction-associated Polypeptide, Zonula Occludens 3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH IF
ZO-3, a ~130kD protein, contains three PSD-95/SAP90, discs-large, ZO-1 (PDZ) domains, a SH3 domain, and a guanylate kinase-liked domain. Like ZO-1 and ZO-2, ZO-3 contains a C-terminal acidic domain and a basic region (between the first and second PDZ domains), making it belongs to the MAGUK protein family. By Western blot analysis, ZO-3 is identified as a ~130kD protein that directly interacts with tight junction proteins ZO-1 and occuldin, but not with ZO-2. However, in one study, ZO-2, ZO-3 and Occludin colocalized with actin aggregates at cell border in MDCK cells. ZO-3 is localized to sites of cell-cell interaction, identical to that of ZO-1 in tight junctions. By immunoprecipitation analysis, ZO-3 co-immunoprecipitates with ZO-1. Using MDCK cells, ZO-3 protein colocalized with ZO-1 by immunofluorescence. In vitro binding assays, the N-terminal of ZO-3 showed preferably binding of ZO-1 and actin filaments3. Moreover, the binding of ZO-1/ZO-2/ZO-3 was also found to interact with certain Claudin proteins.
Catalog #Z0773
ApplicationsSuitable for use in Immunoflourescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence: 0.5-2ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunoprecipitation: 2-3ug
Immunohistochemistry: 4-5ug/ml. Fixation of tissues with 2% paraformaldehyde/picric acid followed by NO post-fixation is required to produce staining in mouse liver tissues. Stronger fixation has been observed to abolish labeling.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminus of the human ZO-3 protein.
SpecificityRecognizes the human ZO-3 protein. MW
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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