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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -ZO-3, Mid (Tight Junction-associated Polypeptide, Zonula Occludens 3)

Anti -ZO-3, Mid (Tight Junction-associated Polypeptide, Zonula Occludens 3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IF
ZO-3, a ~130kD protein, contains three PSD-95/SAP90, discs-large, ZO-1 (PDZ) domains, a SH3 domain, and a guanylate kinase-liked domain. Like ZO-1 and ZO-2, ZO-3 contains a C-terminal acidic domain and a basic region (between the first and second PDZ domains), making it belongs to the MAGUK protein family1. By Western blot analysis, ZO-3 is identified as a ~130kDa protein that directly interacts with tight junction proteins ZO-1 and occuldin, but not with ZO-2. However, in one study, ZO-2, ZO-3 and Occludin colocalized with actin aggregates at cell border in MDCK cells2. ZO-3 is localized to sites of cell-cell interaction, identical to that of ZO-1 in tight junctions. By immunoprecipitation analysis, ZO-3 co-immunoprecipitates with ZO-1. Using MDCK cells, ZO-3 protein colocalized with ZO-1 by immunofluorescence. In vitro binding assays, the N-terminal of ZO-3 showed preferably binding of ZO-1 and actin filaments3. Moreover, the binding of ZO-1/ZO-2/ZO-3 was also found to interact with certain Claudin proteins4.
Catalog #Z0774
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence: 0.5-2ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunoprecipitation: 2-3ug
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the mid region of the human ZO-3 protein.
SpecificityRecognizes human ZO-3 protein with a molecular weight of ~130kD. Crossreactivity with other Zonula Occluden family members has not been observed. Reactivity is con rmed with human T84, HeLa, and canine MDCK cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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