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Abs to Connexin, Junction Proteins
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Anti -ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1)
Anti -ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
B IP IH IF |
|
| Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. | | | Catalog # | Z0775G | | Applications | | | Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 0.5ug/ml detected Z0-1 in 10ug of mouse brain lysate. | | Optimal dilutions to be determined by the researcher. | | Recommended Positive Control | Mouse brain lysate, mouse liver tissue lysate | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2ak | | Clone No | 10H16 | | Host | Rat | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Purified protein corresponding to mouse ZO-1 (NP_033412). | | Specificity | Recognizes mouse ZO-1. Species Species Sequence Homology: rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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