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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1)

Anti -ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IP IH
Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
Catalog #Z0775G
Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.5ug/ml detected Z0-1 in 10ug of mouse liver tissue lysate.
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlMouse liver tissue lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No10H16
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified protein corresponding to mouse ZO-1 (NP_033412). Species Sequence Homology: rat
SpecificityRecognizes mouse ZO-1, ~220kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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