Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution
ELISA: 1:100-1:5000. Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating. Western Blot: 1:100-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Eggshell proteins (zona radiata and zona pellucida) from Atlantic salmon.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 12mM sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes zona radiata proteins in plasma samples from Atlantic salmon. Also recognizes zona radiata proteins from other fish: rainbow trout, arctic char, turbot, cod.
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NCBI
UniProt
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