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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IH

Plasma membrane calcium-ATPase 4 (PMCA4) expression shows little variation across eight drug-treated cell lines and was found to be superior to GAPDH and HPRT1, commonly used reference genes. PMCA4 can be used as a reference gene for normalizing ABC transporter expression in a drug-resistant lung carcinoma cell line. PMCA4 is a good housekeeping gene for normalization of gene expression for polytopic membrane proteins including transporters and receptors.
Catalog #	a4000-50D
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: 1:15,000
	Western Blot: 1:1000
	Immunohistochemistry (Frozen & Paraffin): 1:100-1:500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8J323
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Purity	Purified
Immunogen	Purified human erythrocyte PMCA ATPase. Epitope: aa51-75. This sequence is common to both PMCA4a and 4b ATPase splice variants.
Specificity	Recognizes human PMCA4 4 isoforms, 4a and 4b, at ~133 and 129kD, respectively. Species Crossreactivity: porcine and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.