The Noradrenaline (NE) BioAssay™ ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NE in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
61.7-5000pg/ml
Kit Components
*027181A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use. *027181B: Standard (Lyophilized), 2x1vial 027181C: Standard Diluent, 1x20ml *027181D: Detection Reagent A (green), 1x120ul *027181E: Detection Reagent B (red), 1x120ul 027181F: Assay Diluent A, 1x12ml 027181G: Assay Diluent B, 1x12ml 027181H: TMB Substrate, 1x9ml *027181J: Positive Control, 705.8pg/ml (Lyophilized), 1x1vial 027181K: Stop Solution, 1x6ml 027181L: Wash Buffer, 30X, 1x20ml
Precaution
The Stop Solution (027181K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability
Store *027181A, *027181B, *027181D, *027181E and *027181J at -20°C. After reconstitution of *0271818, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 027181J - Positive Control
Intended Use
The control vials are from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics
The control vials contain human NE in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.
Expected Value
705.8pg/ml
Usage
Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary
1. Prepare all reagents, samples and standards. 2. Add 50ul standard or sample to each well. 3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.