0.156-10ng/ml

0.053ng/ml

<10%

<12%

*027656A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *027656B: Standard, 2x1vial 027656C: Standard Diluent, 1x20ml *027656D: Detection Reagent A, 1x120ul *027656E: Detection Reagent B, 1x120ul 027656F: Assay Diluent A, 1x12ml 027656G: Assay Diluent B, 1x12ml 027656H: TMB Substrate, 1x9ml 027656K: Stop Solution, 1x6ml 027656L: Wash Buffer, 30X, 1x20ml

The Stop Solution (027656K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Store *027656A, *027656B, *027656D and *027656E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.