0.156-10ng/ml

0.051ng/ml

Intra-Assay: CV<10% Inter-Assay: CV<12%

The microtiter plate provided in this kit has been pre-coated with an antibody specific to RUNX2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RUNX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RUNX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RUNX2 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

*027911A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use *027911B: Standard, 2x1vial 027911C: Standard Diluent, 1x20ml *027911D: Detection Reagent A, 1x120ul *027911E: Detection Reagent B, 1x120ul 027911F: Assay Diluent A, 1x12ml 027911G: Assay Diluent B, 1x12ml 027911H: TMB Substrate, 1x9ml 027911K: Stop Solution, 1x6ml 027911L: Wash Buffer, 30x, 1x20ml

Store *027911A, *027911B, *027911D and *027911E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.