Designed for identification of PDE10A inhibitors using fluorescence polarization.

The PDE10A inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE10A enzyme, fluorescently labeled PDE10A substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE10A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE10A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE10A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

PDE10A2: 1x700ng FAM-Cyclic-3’, 5’-AMP: 20uM: 1x50ul PDE assay buffer: 1x12ml Binding Agent: 1x100ul Binding Agent Diluent (cAMP): 1x10ml Black, low binding NUNC black microtiter plate: 1 plate

Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.