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143777 Clusterin BioAssay™ ELISA Kit, Mouse

Specifications
References
Brand
BioAssay™
Gene ID
12759
Kit Type
Sandwich ELISA
Tests
96
Sensitivity
< 10 pg/ml
Detection Method
Colorimetric
Detection Range
312pg/ml-20,000pg/ml
Sample Matrix
cell culture supernates, serum and plasma (heparin, EDTA)
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Sandwich ELISA quantitative detection kit for Mouse Clusterin in cell culture supernates, serum and plasma (heparin, EDTA).

Clusterin (apolipoprotein J) is a 75 - 80kD disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.
Assay Range
312pg/ml-20,000pg/ml
Sensitivity
< 10 pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Uniprot ID
Q06890
Entrez Gene ID
12759
Kit Components
96-well plate pre-coated with anti-Clusterin, Mouse Lyophilized recombinant Clusterin, Mouse standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
References
1. Jones SE, Jomary C (May 2002). "Clusterin". Int. J. Biochem. Cell Biol. 34 (5): 427–431.|2. "Entrez Gene: clusterin".|3. Birkenmeier, E. H., Letts, V. A., Frankel, W. N., Magenheimer, B. S., Calvet, J. P. Sulfated glycoprotein-2 (Sgp-2) maps to mouse chromosome 14. Mammalian Genome 4: 131-132, 1993. |4. Han, B. H., DeMattos, R. B., Dugan, L. L., Kim-Han, J. S., Brendza, R. P., Fryer, J. D., Kierson, M., Cirrito, J., Quick, K., Harmony, J. A. K., Aronow, B. J., Holtzman, D. M. Clusterin contributes to caspase-3-independent brain injury following neonatal hypoxia-ischemia. Nature Med. 7: 338-343, 2001. |
USBio References
No references available
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