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143784 CSF1R/M-CSFR BioAssay™ ELISA Kit, Human

Specifications
References
Brand
BioAssay™
Gene ID
1436
Kit Type
Sandwich ELISA
Tests
96
Sensitivity
< 10 pg/ml
Detection Method
Colorimetric
Detection Range
62.5pg/ml-4000pg/ml
Sample Matrix
cell culture supernates, serum and plasma (heparin, EDTA)
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Sandwich ELISA quantitative detection kit for Human CSF1R/M-CSFR in cell culture supernates, serum and plasma (heparin, EDTA).

CSF1R(Colony stimulating factor 1 receptor),also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on long arm of chromosome 5 (5q32) on the Crick (minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.
Assay Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10 pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Uniprot ID
P07333
Entrez Gene ID
1436
Kit Components
96-well plate pre-coated with anti-CSF1R/M-CSFR, Human Lyophilized recombinant CSF1R/M-CSFR, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
References
1. Galland F, Stefanova M, Lafage M, Birnbaum D (1992). "Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15-q23". Cytogenet. Cell Genet. 60 (2): 114–6.|2. Tamimi RM, Brugge JS, Freedman ML, Miron A, Iglehart JD, Colditz GA, Hankinson SE (January 2008).|3. Pollard JW, Hennighausen L (September 1994). "Colony stimulating factor 1 is required for mammary gland development during pregnancy". Proc. Natl. Acad. Sci. U.S.A. 91 (20): 9312–6. |4. Sapi E (January 2004). "The role of CSF-1 in normal physiology of mammary gland and breast cancer: an update". Exp. Biol. Med. (Maywood) 229 (1): 1–11.|
USBio References
No references available
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