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143984 MMP-9 BioAssay™ ELISA Kit, Mouse

Specifications
References
Brand
BioAssay™
Gene ID
17395
Kit Type
Sandwich ELISA
Tests
96
Sensitivity
< 20 pg/ml
Detection Method
Colorimetric
Detection Range
156pg/ml-10,000pg/ml
Sample Matrix
cell culture supernates, serum and plasma (heparin)
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Sandwich ELISA quantitative detection kit for Mouse MMP-9 in cell culture supernates, serum and plasma (heparin).

The 92kD type IV collagenase is also known as 92kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92kD type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion.2 Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture.3 MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells.4 The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.5
Assay Range
156pg/ml-10,000pg/ml
Sensitivity
< 20 pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Uniprot ID
P41245
Entrez Gene ID
17395
Kit Components
96-well plate pre-coated with anti-MMP-9, Mouse Lyophilized recombinant MMP-9, Mouse standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
References
1. Collier, I. E.; Bruns, G. A. P.; Goldberg, G. I.; Gerhard, D. S. On the structure and chromosome location of the 72- and 92-kDa human type IV collagenase genes. Genomics 9: 429-434, 1991. |2. Turner, H. E.; Nagy, Z.; Esiri, M. M.; Harris, A. L.; Wass, J. A. H. Role of matrix metalloproteinase 9 in pituitary tumor behavior. J. Clin. Endocr. Metab. 85: 2931-2935, 2000. |3. Zhang, B.; Ye, S.; Herrmann, S.-M.; Eriksson, P.; de Maat, M.; Evans, A.; Arveiler, D.; Luc, G.; Cambien, F.; Hamsten, A.; Watkins, H.; Henney, A. M. Functional polymorphism in the regulatory region of gelatinase B gene in relation to severity of coronary atherosclerosis. Circulation 99: 1788-1794, 1999. |4. Coussens, L. M.; Tinkle, C. L.; Hanahan, D.; Werb, Z. MMP-9 supplied by bone marrow-derived cells contributes to skin carcinogenesis. Cell 103: 481-490, 2000. |5. Minematsu, N.; Nakamura, H.; Tateno, H.; Nakajima, T.; Yamaguchi, K. Genetic polymorphism in matrix metalloproteinase-9 and pulmonary emphysema. Biochem. Biophys. Res. Commun. 289: 116-119, 2001. |
USBio References
No references available
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