2.47-200ng/ml

0.92ng/ml

<10%

<12%

*254324A: Microtiter Strips, 1x48 wells or 1x96 wells *254324B: Standard, 2x1 vial 254324C: Standard Diluent, 1x10ml or 1x20ml *254324D: Detection Reagent A (green), 1x60ul or 1x120ul *254324E: Detection Reagent B(red), 1x60ul or 1x120ul 254324F: Assay Diluent A, 1x6ml or 1x12ml 254324G: Assay Diluent B, 1x6ml or 1x12ml 254324H: TMB Substrate, 1x4.5ml or 1x9ml *254324J: Positive Control 65.7ng/ml, 1x1vial 254324K: Stop Solution, 1x3ml or 1x6ml 254324L: Wash Buffer, 30X, 1x10ml or 1x20ml

The Stop Solution (254324K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Store *254324A, *254324B, *254324D, *254324E and *254324J at -20°C. After reconstitution of *254324J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Prepare all reagents, samples and standards. 2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C. 3. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 5. Aspirate and wash 5 times. 6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C. 7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.