Relaxin-3 is a neuropeptide which is highly conserved in species ranging from flies, fish, rodents and humans. It is a member and ancestral gene of the relaxin family of peptides, which includes the namesake hormone relaxin (designated 'H2 relaxin' in humans) which mediates peripheral actions during pregnancy and which was found to relax the pelvic ligament in guinea pigs almost a century ago. The broad distribution of relaxin-3 fibres/RXFP3 within several key neuronal circuits suggests an ability to modulate a broad range of behaviours. This has been confirmed in a growing number of rodent studies, which demonstrate relaxin-3 is able to modulate arousal, the response to stress, feeding/metabolism and memory; and likely plays a role in the generation/regulation of hippocampal theta rhythm.
Sample Type
Cell culture supernates, serum and plasma (heparin)
Intended Use
For quantitative detection of human Relaxin 3 in cell culture supernates, serum and plasma( heparin).
Specificity
Natural and recombinant human Relaxin 3
Test Principle
This human Relaxin 3 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Relaxin 3 has been precoated onto 96-well plates. Standards(E. coli, D119-C142 (A) & A27-R53(B)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Relaxin 3 amount of sample captured in plate.
Kit Components
96-well plate precoated with human Relaxin 3 antibody, 1 Lyophilized recombinant human Relaxin 3 standard, 10ng/tube×2 Biotinylated human Relaxin 3 antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.