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353053 Stem Cell Factor (SCF), BioAssay™ ELISA Kit (Rat)

Specifications
Brand
BioAssay™
Gene ID
60427
Kit Type
Sandwich ELISA
Tests
96
Sample Volume
50-100ul
Sensitivity
<10pg/ml
Detection Method
Colorimetric
Detection Range
31.2-2000pg/ml
Sample Matrix
Cell culture supernates, serum and plasma (EDTA).
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.

Sample Type
Cell culture supernates, serum and plasma (EDTA).
Intended Use
For quantitative detection of rat SCF in cell culture supernates, serum and plasma(EDTA).
Sensitivity
<10pg/ml
Range
31.2-2000pg/ml
Specificity
Natural and recombinant rat SCF
Test Principle
This rat SCF BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SCF has been precoated onto 96-well plates. Standards (E.coli) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat SCF amount of sample captured in plate.
Kit Components
96-well plate precoated with rat SCF antibody, 1 Lyophilized recombinant rat SCF standard, 10ng/tube×2 Biotinylated rat SCF antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Rat
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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