78.125-5000pg/ml

46.875pg/ml

Intra-Assay CV: <8% Inter-Assay CV: <10%

The microtiter plate provided in this kit has been pre-coated with an antibody specific to AB40. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to AB40. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AB40, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AB40 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

*353585A: Microtiter Strips, 1x48 wells or 1x96 wells (8x12 wells) *353585B: Standard, 1x1vial or 2x1vials 353585C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml 353585D: Antibody-Biotin Concentrate, 1x60ul or 1x120ul 353585E: Antibody Dilution Buffer, 1x5ml or 1x10ml 353585F: Streptavidin-HRP Conjugate (SABC), 1x60ul or 1x120ul 353585G: SABC Dilution Buffer, 1x5ml or 1x10ml 353585H: TMB Substrate, 1x5ml or 1x10ml 353585J: Stop Solution, 1x10ml 353585K: Wash Buffer, 25X, 1x15ml or 1x30ml

Store unopened *353585A at 4ºC; store at -20ºC once opened. Store unopened *353585B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Wash plate 2 times before adding standards, samples and control (zero) to wells. 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times. 3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results.