2.344-150mIU/ml

<1.406mIU/ml

Intra-assay CV: <8% Inter-assay CV: <10%

*355751A: Microtiter Strips, 1x96 wells (8x12 wells). *355751B: Standard, 2x1 vial 355751C: Sample/Standard Dilution Buffer, 1x20ml 355751D: Antibody (Biotin) (Concentrated), 1x60ul 355751E: Antibody Dilution Buffer, 1x10ml 355751F: Streptavidin (HRP) (SABC), 1x120ul 355751G: SABC Dilution Buffer, 1x10ml 355751H: TMB Substrate, 1x10ml 355751J: Stop Solution, 1x10ml 355751K: Wash Buffer, 25X, 1x30ml

Store unopened *355751A at 4ºC; store at -20ºC once opened. Store unopened *355751B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

This ELISA kit employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with FSH. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to FSH. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FSH, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FSH in the sample is then determined by comparing the O.D. of the sample to the standard curve.

1. Wash plate 2 times before adding standards and samples to wells. 2. Add 50ul standard or sample to each well. 3. Immediately add 50ul Antibody-Biotin working solution to each well and incubate for 45 minutes at 37°C 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 15-20 minutes at 37°C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

 All products should be handled by qualified personnel only, trained in laboratory procedures.