15.625-1000pg/ml

<9.375pg/ml

Intra-Assay CV: <8% Inter-Assay CV: <10%

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PKC. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to PKC. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PKC, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PKC in the sample is then determined by comparing the O.D. of the sample to the standard curve.

*358152A: Microtiter Strips, 1x96 wells (8x12 wells) *358152B: Standard, 2x1 vial 358152C: Sample/Standard Dilution Buffer, 1x20ml 358152D: Antibody-Biotin (Concentrated), 1x120ul 358152E: Antibody Dilution Buffer, 1x10ml *358152F: Streptavidin-HRP Conjugate (SABC), 1x120ul 358152G: SABC Dilution Buffer, 1x10ml *358152H: TMB Substrate, 1x10ml 358152J: Stop Solution, 1x10ml 358152K: Wash Buffer, 25X, 1x30ml

Store unopened *358152A at 4ºC; store at -20ºC once opened. Store unopened *358152B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store *358152F and *358152H at 4°C; protect from light. Store all other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Wash plate 2 times before adding standards, samples and control (zero) to wells. 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times. 3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results.