USBio Logo

366588 Surfactant Associated Protein C (SPC) BioAssay™ ELISA Kit (Mouse)

Specifications
Brand
BioAssay™
Kit Type
Sandwich ELISA
Tests
96
Sample Volume
100ul
Sensitivity
<0.241ng/ml
Detection Method
Colorimetric
Detection Range
0.625-40ng/ml
Sample Matrix
Tissue homogenates, cell lysates and other biological fluids.
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

The Surfactant Associated Protein C BioAssay™ ELISA Kit (Mouse) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SPC in mouse tissue homogenates, cell lysates and other biological fluids.

Detection Range
0.625-40ng/ml
Sensitivity
<0.241ng/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Test Principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SPC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPC in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Kit Components
*366588A: Microtiter Plate, 1x96 wells. Pre-coated; ready to use. *366588B: Standard, 2x1vial 366588C: Standard Diluent, 1x20ml *366588D: Detection Reagent A, 1x120ul *366588E: Detection Reagent B, 1x120ul 366588F: Assay Diluent A, 1x12ml 366588G: Assay Diluent B, 1x12ml 366588H: TMB Substrate, 1x9ml 366588K: Stop Solution, 1x6ml 366588L: Wash Buffer, 30X, 1x20ml
Storage and Stability
Store *366588A, *366588B, *366588D and *366588E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary
1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.
Source
Mouse
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved