DNA testing is the method of choice for laboratories wishing to produce fast, accurate speciation data. Primerdesign offers multiple solutions for research and applied speciation testing. All of our kits support the detection of low levels of adulterating meat. This is achieved using mitochondrial DNA as a target to enable the highest degree of sensitivity. The new Primerdesign “EASY” and “Advanced” speciation formats contain additional components to help maximize speciation accuracy.
Primerdesign’s Advanced and Easy speciation kits are supplied in a multiplex format enabling both species-specific and universal meat detection in a single well. The relative signals reported by each of these assays produces speciation percentages with minimal well usage. Furthermore, the unique application of a positive control sample as a PCR calibrator allows users to apply recognized relative qPCR calculations to increase the accuracy of their reported results.
Product features
Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to <100 copies of target Accurate controls to confirm findings
Kit Components
Species specific/Universal meat primer/probe mix Species specific - FAM labelled, Universal meat - VIC labelled Positive control template RNAse/DNAse free water
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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