Serum, plasma and other biological fluids.

Competitive ELISA, Coated with Antigen

<69.748ng/ml

78.125-5000ng/ml

Human

This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with AA. During the reaction, AA in the sample or standard competes with a fixed amount of AA on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to AA. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microtiter Strips, 1x48 wells or 1x96 wells Standard, 1x1 vial or 2x1 vials Sample/Standard Dilution Buffer, 1x10ml or 1x20ml Antibody (Biotin) (Concentrated), 1x30ul or 1x60ul Antibody Dilution Buffer, 1x5ml or 1x10ml Streptavidin (HRP) (SABC), 1x60ul or 1x120ul SABC Dilution Buffer, 1x5ml or 1x10ml TMB Substrate, 1x5ml or 1x10ml Stop Solution, 1x5ml or 1x10ml Wash Buffer, 25X, 1x15ml or 1x30ml

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.