31.2-2000pg/ml

<18.75pg/ml

Intra-Assay CV: <8% Inter-Assay CV: <10%

The microtiter plate provided in this kit has been pre-coated with GABA. GABA in the standard or sample competes with a fixed amount of plate-coated GABA for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of GABA in the samples is determined by an inverse correlation between GABA in the sample and the assay signal intensity.

*369916A: Microtiter Strips, 1x48 wells or 1x96 wells. *369916B: Standard, 1x1vial or 2x1 vial 369916C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml 369916D: Antibody-Biotin (Concentrated), 1x30ul or 1x60ul 369916E: Antibody Dilution Buffer, 1x5ml or 1x10ml 369916F: Streptavidin (HRP) (SABC, 1x60ul or 1x120ul 369916G: SABC Dilution Buffer, 1x5ml or 1x10ml 369916H: TMB Substrate, 1x5ml or 1x10ml 369916J: Stop Solution, 1x5ml or 1x10ml 369916K: Wash Buffer, 25X, 1x15ml or 1x30ml

Store unopened *369916A at 4ºC; store at -20ºC once opened. Store unopened *369916B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Wash plate 2 times before adding standards and samples to wells! 2. Add 50ul standard or sample to each well. 3. Add 50ul Antibody (Biotin) working solution to each well and incubate for 45 minutes at 37°C 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 15-20 minutes at 37°C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results