0.313-20ng/ml

0.188ng/ml

Intra-Assay: CV<8% Inter-Assay: CV<10%

The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFN-gamma R2. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to IFN-gamma R2. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFN-gamma R2, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IFN-gamma R2 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

*369992A: Microtiter Strips, 1x48wells or 1x96 wells *369992B: Standard, 1x1vial or 2x1 vial 369992C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml 369992D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul 369992E: Antibody Dilution Buffer, 1x5ml or 1x10ml 369992F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul 369992G: SABC Dilution Buffer, 1x5ml or 1x10ml 369992H: TMB Substrate, 1x5ml or 1x10ml 369992J: Stop Solution, 1x5ml or 1x10ml 369992K: Wash Buffer, 25X, 1x15ml or 1x30ml

Store unopened *369992A at 4ºC; store at -20ºC once opened. Store unopened *369992B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Wash plate 2 times before adding standards and samples to wells! 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times. 3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results