4.688-300IU/ml

2.813IU/ml

Intra-Assay CV: <8% Inter-Assay CV: <10%

This kit was based on indirect enzyme-linked immune-sorbent assay technology. Antigen was pre-coated onto the 96-well plate. The biotin conjugated antibody was used as the detection antibody. The standards and test samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to wells. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of target antigen in the sample is positively correlated with OD450 and can be calculated by plotting the standard curve.

*370205A: Microtiter Strips, 1x48 wells or 1x96 wells **370205B: Standard, 1x1vial or 2x1vial 370205C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml 370205D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul 370205E: Antibody Dilution Buffer, 1x5ml or 1x10ml 370205F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul 370205G: SABC Dilution Buffer, 1x5ml or 1x10ml 370205H: TMB Substrate, 1x5ml or 1x10ml 370205J: Stop Solution, 1x5ml or 1x10ml 370205K: Wash Buffer, 25X, 1x15ml or 1x30ml

Store unopened *370205A at 4ºC; store at -20ºC once opened. Store unopened *370205B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Wash plate 2 times before adding standards and samples to wells! 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times. 3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results

Human

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.