Gentamicin (GEN) belongs to aminoglycoside antibiotics, playing the main role in gram-negative bacteria. It is widely used in veterinary clinical and animal feed additives for its broad antibacterial spectrum, well curative effects and low cost. But it easily causes toxicant side effect during application process, especially in large and non-regulate using, causing Gentamicin residues exceeded in animal derived food, constituting a risk to human health, cranial nerve, hearing and kidney damage. Current methods of detection Gentamicin, such as HPLC or GC-MS, are with sample preparation complex, expensive, difficult and time-consuming. While using the Gentamicin ELISA BioAssay™ kit is both rapidly and accurately detect Gentamicin residues in samples. This BioAssay™ kit is a detection product developed based on ELISA technology, with operation time as short as 50 minutes.
This Gentamicin (GEN) ELISA BioAssay™ Kit is a competitive ELISA intended for the quantitative determination of Gentamicin in milk and tissue (porcine, liver, chicken).
Detection Range
0.3-24.3ppb
Precision
Intra-assay %CV: <10% Inter-assay %CV: <10%
Specificity
Crossreaction rate: Gentamicin: 100%, Kanamycin, Streptomycin sulphate, Neomycin: <0.1%
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gentamicin antigen. Standards or samples are added to the appropriate microtiter plate wells with Gentamicin specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Gentamicin and Gentamicin in standards or samples with the Gentamicin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Gentamicin in the standards or samples. The color development is stopped and the intensity of the color is measured.
Kit Components
376902A: Microtiter Strips, 1x48 wells or 1x96 wells 376902B: Standard 0ppb, 1x500ul or 1x1ml 376902C: Standard 0.3ppb, 1x500ul or 1x1ml 376902D: Standard 0.9ppb, 1x500ul or 1x1ml 376902E: Standard 2.7ppb, 1x500ul or 1x1ml 376902F: Standard 8.1ppb, 1x500ul or 1x1ml 376902G: Standard 24.3ppb, 1x500ul or 1x1ml 376902H: Gentamicin Antibody, 1x3.5ml or 1x7ml 376902J: HRP Conjugate, 1x3.5ml or 1x7ml 376902K: Sample Diluent, 1x10ml or 1x20ml 376902L: Wash Buffer, 10X, 1x15ml or 1x30ml 376902M: TMB Substrate, 1x6ml or 1x12ml 376902N: Stop Solution, 1x5ml or 1x10ml
Note: The Stop Solution (376902H) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability
Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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