USBio Logo

376903 Kanamycin (KA) ELISA BioAssay™ Kit

Specifications
Brand
BioAssay™
Kit Type
Competitive ELISA
Tests
4896
Sensitivity
0.5ppb
Detection Method
Colorimetric
Detection Range
0.5-40.5ppb
Sample Matrix
Milk, milk powder, tissue (chicken, porcine).
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C

Kanamycin is an aminoglycoside antibiotic and is widely used in treating animal diseases. It harms the 8th cranial nerves, causing damages to the vestibular and cochlear. The main manifestations of renal toxicity are the damages of the proximal convoluted tubule, causing protein urine, hematuresis, renal hypofunction, etc. The residues of Kanamycin in animal derived food affect human health, in China and Occident, Kanamycin has been limited to use for its neurotoxicity and nephrotoxicity. This BioAssay™ kit is a detection product developed based on ELISA technology, with operation time as short as 50 minutes.

The Kanamycin (KA) ELISA BioAssay™ Kit is a competitive ELISA intended for the in vitro quantitative determination of Kanamycin concentrations in milk, milk powder, tissue (chicken, porcine).
Detection Range
0.5-40.5ppb
Sensitivity
0.5ppb
Precision
Intra-assay CV%: <10% Inter-assay CV%: <10%
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kanamycin antigen. Standards or samples are added to the appropriate microtiter plate wells with Kanamycin specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Kanamycin and Kanamycin in standards or samples with the Kanamycin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Kanamycin in the standards or samples. The color development is stopped and the intensity of the color is measured.
Kit Components
376903A: Microtiter Strips, 1x48 wells or 1x96 wells 376903B: Standard 0ppb, 1x500ul or 1x1ml 376903C: Standard 0.5ppb, 1x500ul or 1x1ml 376903D: Standard 1.5ppb, 1x500ul or 1x1ml 376903E: Standard 4.5ppb, 1x500ul or 1x1ml 376903F: Standard 13.5ppb, 1x500ul or 1x1ml 376903G: Standard 40.5ppb, 1x500ul or 1x1ml 376903H: Kanamycin Antibody, 1x3.5ml or 1x7ml 376903J: HRP Conjugate, 1x3.5ml or 1x7ml 376903K: TMB Substrate, 1x6ml or 1x12ml 376903L: Sample Diluent, 1x10ml or 1x20ml 376903M: Wash Buffer, 10X, 1x25ml or 1x50ml 376903N: Stop Solution, 1x5ml or 1x10ml
Note: The Stop Solution (376903N) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability
Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved