Ochratoxin A (OTA) is a toxic metabolite produced by Aspergillus flavus and Penicillium genera. Ochratoxin A has a strong liver and kidney toxicity, and has teratogenicity, mutagenicity and carcinogenicity. Ochratoxin A was considered to be the second important and harmful mycotoxin in the family following with Aflatoxin flavus. As the Ochratoxin A producing fungi exist widely in nature, especially higher yield in cereals and cereals products, coffee, cocoa contaminated with Ochratoxin A, it has been potential threat for human health and development of animal husbandry. This kit is a detection product developed based on ELISA technology, with operation time as short as 50 minutes.
Sample Type
Feed 5ppb, soybean 5ppb
Intended Use
This BioAssay™ kit allows for the in vitro quantitative determination of Ochratoxin A concentrations in feed, soybean meal.
Precision
Intra-assay: CV%<10% Inter-assay: CV%<10%
Test Principle
This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay™ kit has been pre-coated with Ochratoxin A antigen. Standards or samples are added to the appropriate microtiter plate wells with Ochratoxin A specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Ochratoxin A and Ochratoxin A in standards or samples with the Ochratoxin A special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Ochratoxin A in the standards or samples. The color development is stopped and the intensity of the color is measured.
Kit Components
Assay plate, 96T/48T Standard (96T), 6x1ml Standard (48T), 6x0.5ml HRP-conjugate (96T), 1x7ml HRP-conjugate (48T), 1x3.5ml Antibody (96T), 1x7ml Antibody (48T), 1x1x3.5ml TMB Substrate (96T), 1x12ml TMB Substrate (48T), 1x6ml Stop Solution (96T), 1x10ml Stop Solution, (48T), 1x5ml Sample Diluent (20X) (96T), 1x20ml Sample DIluent (20X) (48T), 1x10ml Wash Buffer (10X) (96T), 1x50ml Wash Buffer (10X) (48T), 1x25ml Adhesive Strip (96T/48T), 4x strips Instruction Manual (96T/48T), 1x manual
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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