T-2 toxin is mycotoxin produced by several Fusarium species. Food crops as wheat, barely, corn, and their products are mainly contaminated. T-2 toxin is a threat for human health and animal husbandry. T-2 toxin primarily affects the blood, liver, kidneys, pancreas, muscles and function of the lymphocyte. The general symptoms of T-2 toxin poisoning are anorexia, vomiting, diarrhea, growth retardation, reproduction and neurological dysfunction. This BioAssay™ kit is a detection product developed based on ELISA technology, with operation time as short as 50 minutes.
Intended Use
This BioAssay™ kit allows for the in vitro quantitative determination of T-2 toxin concentrations in feed.
Precision
Intra-assay: CV%<10% Inter-assay: CV%<10%
Test Principle
This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay™ kit has been pre-coated with T-2 toxin antigen. Standards or samples are added to the appropriate microtiter plate wells with T-2 toxin specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated T-2 toxin and T-2 toxin in standards or samples with the T-2 toxin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of T-2 toxin in the standards or samples. The color development is stopped and the intensity of the color is measured.
Kit Components
Assay plate, 96T/48T Standard (96T), 5x1ml Standard (48T), 5x0.5ml HRP-conjugate (96T), 1x7ml HRP-conjugate (48T), 1x3.5ml Antibody (96T), 1x7ml Antibody (48T), 1x1x3.5ml TMB Substrate (96T), 1x12ml TMB Substrate (48T), 1x6ml Stop Solution (96T), 1x10ml Stop Solution, (48T), 1x5ml Sample Diluent (2x) (96T), 1x20ml Sample DIluent (2x) (48T), 1x10ml Wash Buffer (10X) (96T), 1x50ml Wash Buffer (10X) (48T), 1x25ml Adhesive Strip (96T/48T), 4x strips Instruction Manual (96T/48T), 1x manual
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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