0.05-4.05ppb

0.05ppb

Intra-assay: CV%<10% Inter-assay: CV%<10%

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tetracyclines antigen. Standards or samples are added to the appropriate microtiter plate wells with Tetracyclines specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Tetracyclines and Tetracyclines in standards or samples with the Tetracyclines special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Tetracyclines in the standards or samples. The color development is stopped and the intensity of the color is measured.

376919A: Microtiter Strips, 1x48 wells or 1x96 wells 376919B: Standard 0ppb, 1x500ul or 1x1ml 376919C: Standard 0.05ppb, 1x500ul or 1x1ml 376919D: Standard 0.15ppb, 1x500ul or 1x1ml 376919E: Standard 0.45ppb, 1x500ul or 1x1ml 376919F: Standard 1.35ppb, 1x500ul or 1x1ml 376919G: Standard 4.05ppb, 1x500ul or 1x1ml 376919H: Tetracyclines Antibody, 1x3.5ml or 1x7ml 376919J: HRP Conjugate, 1x3.5ml or 1x7ml 376919K: Substrate A, 1x3.5ml or 1x7ml 376919L: Substrate B, 1x3.5ml or 1x7ml 376919M: Sample Extraction, 10X, 2x50ml 376919N: Wash Buffer, 20X, 1x15ml 376919P: Stop Solution, 1x3.5ml or 7ml

Note: The Stop Solution (376919P) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.