31.25-2000pg/ml

18.75pg/ml

Intra-Assay %CV: <10% Inter-Assay %CV: <10%

Recognizes natural and some recombinant human STUB1. No significant crossreactivity or interference between human STUB1 and analogues was observed.

The microtiter plate provided in this kit has been pre-coated with an antibody specific to STUB1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to STUB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Free components are washed away. The substrate solution is added, only those wells that contain STUB1, biotin-conjugated antibody and enzyme-conjugated Avidin will apear blue in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm ± 2nm. The OD value is proportional to the concentration of human STUB1. The concentration of STUB1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

*384523A: Microtiter Strips, 1x96 wells, Pre-coated; ready to use *384523B: Standard, 2x1vial 384523C: Standard Diluent, 1x20ml *384523D: Biotin Detection, 100X, 1x120ul *384523E: HRP Conjugate, 100X, 1x120ul 384523F: Biotin Detection Diluent, 1x14ml 384523G: HRP Diluent, 1x14ml 384523H: Substrate, 1x10ml 384523K: Stop Solution, 1x10ml 384523L: Wash Buffer, 25X, 1x30ml

The Stop Solution (384523K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Store *384523A, *384523B, *384523D and *384523E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap

1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 90 minutes at 37°C. 3. Aspirate and add 100ul prepared Biotin Working Solution. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul preparedHRP Detection Working Solution. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate. Incubate 15 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.