Serum, plasma and other biological fluids.

For quantitative detection of Septin 9 in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.

<0.188ng/ml

Intra-Assay CV: <8% Inter-Assay CV: <10%

0.313-20ng/ml

Human

This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Septin 9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Septin 9 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Septin 9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Septin 9 can be calculated.

Microtiter Strips, 1x48 wells or 1x96 wells Standard, 1x1 vial or 2x1 vials Sample/Standard Dilution Buffer, 1x10ml or 1x20ml Antibody (Biotin) (Concentrated), 1x30ul or 1x60ul Antibody Dilution Buffer, 1x5ml or 1x10ml Streptavidin (HRP) (SABC), 1x60ul or 1x120ul SABC Dilution Buffer, 1x5ml or 1x10ml TMB Substrate, 1x5ml or 1x10ml Stop Solution, 1x5ml or 1x10ml Wash Buffer, 25X, 1x15ml or 1x30ml

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.